| 1. | Metallothionein - 3 ; yeast two - hybrid system ; interaction ; dimerization
金属硫蛋白- 3酵母双杂交系统相互作用二聚体 |
| 2. | This work provides a good foundation for the establishment of yeast two - hybrid system in our lab
本工作为我室酵母双杂交方法的建立打下了一个良好的基础。 |
| 3. | Detection of interactions among metallothioneins by yeast two - hybrid system detection of interactions among metallothioneins by yeast two - hybrid system
酵母双杂交系统检测金属硫蛋白mt间的相互作用 |
| 4. | 3 fifty candidates proteins possible interacting with the nthk1 protein were screened from the tobacco cdna library through the yeast two - hybrid system
3利用酵母双杂交技术从烟草的cdna表达文库中筛到50个可能与nthk1的相互作用的蛋白基因。 |
| 5. | Further we cloned full - length cdna of the ked like protein coding region via pcr amplification and confirmed its interaction with acam2 in yeast two - hybrid system
进一步通过rt . pcr克隆了ked样蛋白编码区全长cdna ，通过双杂交验证了全长ked样蛋白与acamz的相互作用，结果为阳性。 |
| 6. | 3 . the yeast two - hybrid system has been broadly used to identify protein partners . in recent years , protein - peptide interactions have also been analyzed with this system
随着该方法的不断完善，它不但可用来在体检验蛋白质间的相互作用，而且还能用来研究蛋白质与小肤的相互作用。 |
| 7. | We cloned full - length cdna of ca coding region via pcr amplification , but a negative result was obtained when the interaction of full - length ca and ga was tested in yeast two - hybrid system
通过rt - pcr克隆了ca编码区全长cdna ，但是在双杂交系统检测全长ca与g蛋白的相互作用时，结果为阴性。 |
| 8. | In order to study the upstream and downstream components in g protein and calmodulin signal pathways , a yeast two - hybrid system was used to screen interaction partners of these two proteins
本文利用酵母双杂交系统分别筛选了与这两种蛋白相互作用的蛋白质，以进一步研究g蛋白和cam信号转导途径中的其它上下游组分。 |
| 9. | In order to figure out the machanism of transcription control by ap - 2 a and to find the partner proteins which derectly binding to ap - 2 a , a yeast two - hybrid system by using ap - 2 a as a bait was performed to screen the hela cell cdna library
为了阐明ap - 2确切的转录调控方式，找出与其直接相互作用的蛋白质因子，我们以ap - 2为饵蛋白通过酵母双杂交对hela细胞的cdna文库进行了筛选。 |
| 10. | The yeast two - hybrid system was established . in yeast two - hybrid system , mt - 3 interacts with mt - 3 . a further two - hybrid test also indicated that mt - 3 weakly interacted with its isoform mt - 1 . these primary results suggest that mt - 3 probably trend to homodimerize or heterodimerize in vivo
结果表明： mt - 3与mt - 3之间在酵母细胞内能发生相互作用，进一步用酵母双杂交实验还表明mt - 3与mt - 1间也存在弱相互作用。 |
